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Improvements in the approximate determination of S‐methylcysteine sulphoxide (kale anaemia factor)
Author(s) -
Matheson Norman A.
Publication year - 1980
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740310308
Subject(s) - ninhydrin , extraction (chemistry) , chromatography , chemistry , aqueous solution , ion chromatography , paper chromatography , thin layer chromatography , amino acid , organic chemistry , biochemistry
The S‐methylcysteine sulphoxide in kale samples was determined by ethanolic extraction and thin layer chromatography of the crude extract. The separated compound reacted with ninhydrin giving a colour which was interpolated visually in a series of colours produced by standards run on the same plate. Results by this approximate procedure are compared with those found by exhaustive aqueous extraction and automatic amino acid analysis by ion‐exchange chromatography.