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Simultaneous quantitative determination of sucrose, raffinose and stachyose by invertase hydrolysis and gas—liquid chromatography
Author(s) -
Ford Clive W.
Publication year - 1979
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740300904
Subject(s) - stachyose , raffinose , melibiose , chemistry , fructose , sucrose , invertase , chromatography , hydrolysis , oligosaccharide , gas chromatography , biochemistry
A method for small‐scale ( ca 0.5 mg) quantitative determination of sucrose, raffinose and stachyose is described. After invertase hydrolysis, glucose, fructose and melibiose were estimated by gas‐liquid chromatography of the trimethylsilyl derivatives. Sucrose and raffinose were directly estimated from glucose and melibiose levels, respectively. Stachyose values were calculated from the amounts of fructose remaining after deductions were made for the calculated sucrose and raffinose levels. The accuracy of the method from known saccharide mixtures was 6% for sucrose, 3% for raffinose and 10% for stachyose. The method was also shown to be able to simultaneously determine inositols and inositol galactosides.