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Improved electrophoretic methods for identifying cereal varieties
Author(s) -
Du Cros Diana L.,
Wrigley Colin W.
Publication year - 1979
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740300807
Subject(s) - endosperm , triticale , isoelectric focusing , chromatography , electrophoresis , polyacrylamide gel electrophoresis , starch , chemistry , polyacrylamide , gel electrophoresis , biology , biochemistry , botany , enzyme , polymer chemistry
Several electrophorectic methods are compared in terms of their reliability, convenience and ability to distinguish between varieties of wheat, barley, rye and triticale using proteins extracted from the endosperm with 1M urea. Gradient gel electrophoresis offers an improvement, particularly in convenience, over the use of starch gels and uniform‐pore polyacrylamide. Varietal identification is described using gels having gradients in polyacrylamide concentration of 3‐27% or 2‐16%, both being available commercially ready for use. Electrophoresis in the latter gels at 400 V takes only half an hour for barley and an hour for wheat. In addition, a simple procedure is described for gel isoelectric focusing which, used alone or in combination with gel electrophoresis, provides high resolution of endosperm proteins for distinguishing between closely related varieties.