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Extraction of nitrogenous constituents from leguminosae : Solubility of nitrogen from vicia sativa
Author(s) -
Kazazis Ioannis,
Kalaissakis Pericles
Publication year - 1979
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740300803
Subject(s) - solubility , extraction (chemistry) , nitrogen , chemistry , sodium hydroxide , solvent , molar concentration , vicia sativa , sodium , chromatography , nuclear chemistry , botany , organic chemistry , biology
A study was made of the extraction of nitrogen from vetch seeds ( Vicia sativa ) with solutions of neutral and alkaline salts. Sodium hydroxide extracted most nitrogen and, using a fixed meal: solvent ratio, solubility could be increased by reducing the particle size (dry milling) or increasing the time of extraction and solvent molarity. Stirring was slightly more efficient than shaking. When a fixed concentration of NaOH was used with increasing meal: solvent ratio, the solubility of the nitrogen decreased steadily. Optimum conditions for maximum extraction of nitrogen were established. With sodium chloride minimum solubility of N was found using a concentration of less than 0.01M and maximum with concentrations greater than 0.25M. Extraction with calcium hydroxide solubilised increasing amounts of N up to concentrations of 0.01M, but above this solubility of N decreased.