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The in vitro lipolysis and biohydrogenation of monogalactosyldiglyceride by whole rumen contents and its fractions
Author(s) -
Singh Sudarshan,
Clement Hawke J.
Publication year - 1979
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740300609
Subject(s) - rumen , lipolysis , incubation , hydrolysis , biochemistry , fatty acid , bacteria , biology , fraction (chemistry) , chemistry , food science , chromatography , adipose tissue , fermentation , genetics
The contribution of bacterial, protozoal, participate and cell‐free fractions of rumen fluid to the lipolysis of monogalactolipid and the hydrogenation of its acyl constituents has been examined. Each of these fractions hydrolysed monogalactolipid and free fatty acids were the major acyl products. Almost all the radioactivity added as monogalactolipid to rumen fluid was distributed between the particulate and bacterial fractions and half to two‐thirds of the radioactivity in each of the four fractions was recovered as free fatty acids. Both 16:3 and 18:3, after release from monogalactolipids, were hydrogenated to 16:2 and 18:2 at short incubation times. As incubation was continued there was a rapid decline in dienoic fatty acids and an increase in saturated and monoenoic fatty acids. There was a greater accumulation of trans ‐18: 1 than cis ‐18:1. Only the particulate fraction of rumen fluid gave patterns of hydrogenation similar to that obtained with rumen fluid. Bacteria in free suspension had little ability to hydrogenate trienoic fatty acids beyond the dienoic stage and neither protozoa nor the cell‐free supernatant gave significant biohydrogenation of trienoic fatty acids. On the other hand, the presence of monoenoic and dienoic fatty acids in the bacterial, protozoal and cell‐free fractions isolated from total rumen fluid after incubation with monogalactolipid indicated their transfer to these fractions after biohydrogenation in the particulate fraction.