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Preparation of white leaf protein concentrate using a polyanionic flocculant
Author(s) -
Bray Walter J.,
Humphries Catherine
Publication year - 1979
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740300212
Subject(s) - lolium perenne , flocculation , medicago sativa , chemistry , centrifugation , trifolium repens , polyacrylamide , festuca arundinacea , food science , red clover , chromatography , perennial plant , agronomy , biology , poaceae , organic chemistry , polymer chemistry
Juice was extracted from lucerne ( Medicago sativa L.), tall fescue ( Festuca arundinacea L. var. Alta) and perennial ryegrass ( Lolium perenne L. var. Melle). The removal of pigmented material from the juice, by the destabilisation of the colloidally suspended chloroplastic particles, using a number of flocculants in combination with pH adjustment, was studied. Although the addition of calcium and magnesium ions partially precipitated pigmented material, Superfloc A150, a polyanionic polyacrylamide flocculant, was more effective. pH adjustments, both before and after the addition of this flocculant, led to two possible methods for precipitating completely pigmented material, a one‐stage process where one pH adjustment and centrifugation was used, and a two‐stage process where two pH adjustments and centrifugations were made. These processes allowed the recovery of a ‘white ’protein suitable for use in human food formulations.