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The determination of the component oligosaccharides of lupinseeds by high pressure liquid chromatography
Author(s) -
Macrae Robert,
ZandMoghaddam Ahmad
Publication year - 1978
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740291214
Subject(s) - chromatography , stachyose , chemistry , oligosaccharide , extraction (chemistry) , aqueous solution , high performance liquid chromatography , soy protein , methanol , precipitation , high pressure , raffinose , food science , biochemistry , organic chemistry , sucrose , physics , engineering physics , meteorology , engineering
A simple rapid method is reported for the analysis of oligosaccharides in lupinseeds. The procedure involves extraction of the ground, defatted seeds with aqueous methanol, precipitation of protein with Carrez solution and subsequent analysis using high pressure liquid chromatography. The chromatographic separation utilises Spherisorb amino columns (five micron) packed in our laboratory. Quantification is achieved by direct comparison with aqueous standards. The oligosaccharide composition of ten lupinseed varieties, from four species, are reported and the values obtained compared with those found for soy beans. The contents of stachyose and verbascose, which are considered to be of major importance as flatus factors, are found to be similar to those in soy beans for some lupinseed species (L. albus and L. angustifolius) but considerably higher in others (L. luteus and L. mutabilis) .