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An automated colorimetric method for mimosine in Leucaena leaves
Author(s) -
Megarrity Robert G.
Publication year - 1978
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740290216
Subject(s) - mimosine , leucaena leucocephala , chromatography , leucaena , chemistry , hydrochloric acid , extraction (chemistry) , botany , biology , organic chemistry
Simplified extraction and colorimetric techniques have been developed for the determination of the toxic amino acid mimosine in Leucaena leucocephala. Fresh leaf material is extracted with dilute hydrochloric acid containing activated carbon and the concentration of mimosine in the clarified extract determined with an Auto‐Analyser. Satisfactory recoveries of added mimosine were obtained, and the results were in agreement with a manual method which was based on electrophoretic separation.