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A simple and rapid screening method for the determination of protein and tryptophan in kernel halves and small samples of barley meal
Author(s) -
Ahokas Hannu
Publication year - 1978
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740290108
Subject(s) - tryptophan , sodium hydroxide , meal , dilution , chemistry , food science , kernel (algebra) , chromatography , mathematics , biochemistry , amino acid , physics , combinatorics , thermodynamics
The protein of distal halves of barley kernels or small meal samples was extracted with ethanolic sodium hydroxide solution. After dilution, the protein content was estimated with a spectrophotometer by measurements at 215 and 225 nm. The tryptophan content was estimated from the same extract by a spectrophotofluorimeter. The methods showed high correlations with those used for control. The presented methods can be used in screening kernels in segregating ears or selecting single plants.