Premium
An inexpensive procedure for the rapid determination of total nitrogen in multiple biological samples
Author(s) -
SylvesterBradley Roger,
Howitt Stephen G.
Publication year - 1977
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740280315
Subject(s) - kjeldahl method , indophenol , nitrogen , chemistry , chromatography , ammonia , digestion (alchemy) , environmental chemistry , biochemistry , organic chemistry
A method is described for rapid, total nitrogen determinations on samples of biological materials. A modification of the indophenol blue colour reaction is used to determine ammonia released by a semi‐micro Kjeldahl digestion method which uses equipment which is cheap to construct. Using the procedure one worker can analyse 40 samples per hour. Recovery of nitrogen from a standard lysine solution was 98.3 %.