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Use of Aspergillus flavus to evaluate the relative nutritive value in cultivars of rye, wheat and triticale
Author(s) -
Mohyuddin Mirza,
Sharma Trilok R.,
Kaul Avtar K.,
Niemann Ernst G.
Publication year - 1976
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740271009
Subject(s) - triticale , mycelium , starch , food science , biomass (ecology) , chemistry , aspergillus flavus , cultivar , lysine , carbohydrate , maize starch , agronomy , biology , botany , horticulture , biochemistry , amino acid
Abstract Aspergillus flavus Link ex Fr., was used to evaluate the relative nutritive value in a set of breeder's material involving 2 rye, 5 wheat and 33 triticale samples ranging in protein content from 12.6 to 21.9%. The samples were tested at equal nitrogen level. Total dry weight of the mycelium produced after 72 h of shaking was used as the index of biomass. The biomass showed a negative correlation with protein content and dye‐binding capacity, and a positive correlation with protein index and lysine in protein. The fungus was able to pick up an “unusual” sample which had high protein and high lysine content. In addition, the effects of initial inoculum concentration, pH value and excess soluble starch on biomass production were studied. Initial spore concentrations of 10 6 and 10 8 /ml and pH values of 3.5 and 6.2 did not make significant changes in biomass. However, the presence of excess soluble starch increased the mycelial yield without basically altering the results. Lysine dependent strains of Neurospora crassa and Aspergillus nidulans did not grow well in shake cultures and it appeared that the carbohydrate energy source was left unutilised. This “left over” starch was considered to be a possible source of error in biomass determination.