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A simplified procedure for fractionating plant materials
Author(s) -
Laird William M.,
Mbadiwe Eddie I.,
Synge Richard L. M.
Publication year - 1976
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740270206
Subject(s) - chemistry , extraction (chemistry) , chloroform , acetic acid , leafy , medicago sativa , shoot , methanol , solanum tuberosum , polysaccharide , phenol , chromatography , botany , organic chemistry , biology
Sequential extraction of plant materials with methanol–chloroform–water and phenol–acetic acid–water mixtures gave good yields of water‐soluble low‐molecular‐mass substances, lipids, proteins and polysaccharides in separate fractions unlaboriously and with little chemical damage. Results with potato tubers ( Solanum tuberosum L.), leafy shoots of lucerne ( Medicago sativa L.) and an oilseed ( Pentaclethra macrophylla Benth.) are presented.