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A spectrofluorimetric assay of the tryptophan content of feedstuffs
Author(s) -
Buttery Peter J.,
Soar John B.
Publication year - 1975
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740260905
Subject(s) - tryptophan , fluorophore , sodium hydroxide , chemistry , hydrolysis , chromatography , fluorescence , food science , biochemistry , organic chemistry , amino acid , physics , quantum mechanics
A method for the analysis of the tryptophan content of feedstuffs employing sodium hydroxide hydrolysis followed by the spectrofluorimetric estimation of tryptophan is described. Provided the development of the fluorophore is carried out in the absence of light, satisfactory results for a wide range of feedstuffs are obtained.