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Enzymatic changes associated with ripening of grape berries
Author(s) -
Dal Peruffo A. Belin,
Pallavicini C.
Publication year - 1975
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740260502
Subject(s) - ripening , aminopeptidase , glutamate dehydrogenase , malate dehydrogenase , biochemistry , leucine , isoelectric focusing , enzyme , acid phosphatase , dehydrogenase , chemistry , biology , food science , amino acid , glutamate receptor , receptor
The isoenzyme patterns of glutamate dehydrogenase, malate dehydrogenase, leucine aminopeptidase and acid phosphatase in grape berries at various stages of ripening were determined by gel isoelectric focusing. The ripening of the grape berries was characterised by the appearance of new isoenzyme bands for malate dehydrogenase, acid phosphatase and glutamate dehydrogenase. Evidence of increase in the total enzyme activities during the ripening was obtained. Only one form of leucine aminopeptidase was found, but this also increased in intensity as ripening progressed.