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An approach to the chemical analysis of folic acid: Gas chromatography of a degradation product from synthetic folic acid samples
Author(s) -
Seifert R. M.
Publication year - 1974
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740251211
Subject(s) - chemistry , folic acid , hydrolysis , chromatography , amine gas treating , yield (engineering) , gas chromatography , pteridine , degradation (telecommunications) , organic chemistry , acid hydrolysis , enzyme , medicine , telecommunications , materials science , computer science , metallurgy
A new chemical approach to the assay of synthetic folic acid has been investigated. Folic acid is cleaved with bisulphite to give a pteridine fraction and p ‐amino‐benzoylglutamic acid. Acid hydrolysis and subsequent esterification converted the aromatic amine to ethyl p ‐aminobenzoate. This ester, when analysed by gas chromatography (g.l.c.), gave consistent yields averaging 49% from 50 to 1000 μg of folic acid. The recovery being predictable, the percentage yield can be used as a correction factor for unknown samples. The present procedure can be used for synthetic folic acid.