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Comparison of methods for the determination of available lysine value in animal protein concentrations
Author(s) -
Hussein Laila A.
Publication year - 1974
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740250202
Subject(s) - lysine , reagent , chromatography , chemistry , solvent , methanol , column (typography) , value (mathematics) , mathematics , biochemistry , organic chemistry , amino acid , statistics , geometry , connection (principal bundle)
A comparison was made between Carpenter's, Erbersdobler's and Rao's methods for the evaluation of available lysine in nine different protein concentrates. The use of methanol is not recommended as a solvent for the FDNB reagent. Measurement at 360 nm gives erroneously higher results. The separation of εDNP‐lysine was best on column chromatography.