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Lucerne tannins. III. Metabolic fate of lucerne tannins in mice
Author(s) -
Milić Božidar Lj.,
Stojanović Srdjan
Publication year - 1972
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740231004
Subject(s) - tannin , acetic acid , chemistry , proanthocyanidin , feces , glycine , gallic acid , food science , chromatography , biochemistry , biology , polyphenol , microbiology and biotechnology , amino acid , antioxidant
Metabolites of the alimentary tract of mice fed 0.5 and 4.0% of lucerne tannin isolate in an artificial diet were studied. Twelve identified and four unidentified substances were detected by paper chromatography on Whatman Chromedia paper SG‐81, coated with silica gel, using the solvent system 1‐butanol‐acetic acid‐water. 3‐Methoxy‐4‐hydroxybenzoic acid, 5‐ o ‐methylgallic acid, (–)‐epigallocatechin gallate, (+)‐gallocatechin and (–)‐epicatechin were found in greater amounts and 3,4‐dihydroxycinnamic acid, 3,4‐dimethoxy‐5‐hydroxybenzoic acid, m ‐digallic acid, m ‐hydroxycinnamic‐acid, m ‐hydroxyhippuric acid and (–)‐gallocatechin gallate in smaller ones in faeces of mice fed on the diet containing 0.5 and 4.0% of tannin. These constituents were detected in traces or were not detected at all in faeces of mice fed on the diet without tannins. The main metabolites of the alimentary tract of mice on synthetic diet were m ‐hydroxylcinnamoyl glycine and four unidentified substances.