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Separation and determination of isoflavones in the protein concentrate from red clover leaves
Author(s) -
Glencross R. G.,
Festenstein G. N.,
King H. G. C.
Publication year - 1972
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740230314
Subject(s) - isoflavones , red clover , chromatography , chemistry , sephadex , elution , chlorophyll , aqueous extract , ammonia , botany , biochemistry , biology , traditional medicine , organic chemistry , enzyme , medicine
Oestrogenic isoflavones were extracted from the protein concentrate of red clover leaves, isolated on columns of Sephadex G‐25 using 0.1 M‐ammonia and determined spectrophotometrically in the eluted fractions. Most of the chlorophyll and its breakdown products were precipitated from the original aqueous ethanolic extract before the lipids and then the isoflavones were finally extracted.