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Determination of carbohydrates by liquid chromatography: Lactose in milk
Author(s) -
Hobbs J. S.,
Lawrence J. G.
Publication year - 1972
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740230107
Subject(s) - lactose , chromatography , chemistry , galactose , aqueous solution , elution , column chromatography , ethanol , organic chemistry
A rapid, simple method for the determination of lactose in milk or milk products is described. An internal standard (galactose) is added to the milk which is then extracted with carbon tetrachloride. A sample of the aqueous layer is injected onto a strongly acidic cation exchange column (Li + form, 17 μm particles) and eluted with aqueous ethanol, the eluent composition being monitored with a modified moving‐wire detector. Applications demonstrating the precision and accuracy of the method are described.