Premium
Rapeseed protein fractions: II.—Chemical composition and biological quality of a lipid‐protein concentrate from rapeseed (Brassica napus L.)
Author(s) -
Eklund A.,
Ågren G.,
Langler T.,
Stenram U.,
Nordgren M.
Publication year - 1971
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740221213
Subject(s) - rapeseed , brassica , methionine , biological value , chemistry , cystine , chemical composition , food science , composition (language) , biochemistry , biology , amino acid , enzyme , botany , linguistics , philosophy , organic chemistry , cysteine
Three types (I‐III) of a lipid‐protein concentrate were prepared from rapeseeds ( Brassica napus L.) by an aqueous ethanol extraction method. The lipid‐protein material was washed one (I) or three (II/III) times with hot water and dried either by spray‐drying (I/II) or in a vacuum oven (III). The chemical composition of these products was assayed. Type III had a protein content of 43 % (N × 5.62) and a chemical score of 61. Methionine plus cystine were the first limiting amino acids. The biological value and toxicological properties of the preparations were tested on growing male rats. The protein efficiency ratios ( PER ) for a 3‐week period were 2.1 (I), 2.9 (II) or 2.8 (III). The productive protein value ( PPV , ‘apparent NPU ’) for the same period was 67 (II). Histopathological examinations were carried out on liver, spleen, kidneys, adrenals, testes, heart, lungs, pancreas, intestine, thyroid gland and the hypophysis. The ultrastructure of liver was studied by electron microscopy. The findings were normal and similar to those from the control rats except for higher incidence of renal calculi in the experimental group given the lipid‐protein concentrate type I. Haematological analyses gave normal results.