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Extraction and analysis of free amion acids from sheep digesta
Author(s) -
Peterson P. J.,
Hendtlass Jane,
Macrae J. C.,
Pearce P. D.
Publication year - 1971
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740221013
Subject(s) - ninhydrin , chromatography , amino acid , chemistry , chloroform , extraction (chemistry) , thin layer chromatography , ileum , amino acid analysis , ultrafiltration (renal) , biochemistry
A routine procedure entailing three methanol‐chloroform‐water extractions and an ultrafiltration step was adopted to extract the free amino acids from freeze‐dried sheep digesta. Detailed estimation and idefltification of the amino acids both by thin‐layer electrophoresis (t.l.e,) coupled with thin‐layer chromatography (t.l.e.) and by using a Beckman IZOC analyser was undertaken, Generally, there was agreement between the two methods, the latter being preferred because several amino acids were not suficiently separated by the t.l.e. and t.l.c. methods. The t.l.e. and t.l.c. method, however, showed the presence of several unidentified ninhydrin positive compounds not detected during automatic analyser estimations. Detailed analysis of the digestive tract samples revealed that the free amino acids decreased in amount during passage through the animal. However, five amino acids had higher values in the ileum than in the duodenum.