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The use of disc electrophoresis to evaluate protein changes accompanying exposure of perennial ryegrass ( Lolium perenne L.) to cold hardening conditions
Author(s) -
Draper S. R.,
Watson S. E.
Publication year - 1971
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740221003
Subject(s) - lolium perenne , perennial plant , polyacrylamide gel electrophoresis , electrophoresis , polyacrylamide , cold hardening , chemistry , gel electrophoresis , botany , agronomy , biology , chromatography , biochemistry , polymer chemistry , enzyme
Polyacrylamide gel electrophoresis has been used to separate the soluble proteins of leaves of perennial' ryegrass, harvested before and after exposure to cold hardening conditions. Extracts from plants which had been exposed to low temperatures and short day lengths contained more soluble protein than extracts of non‐hardened plants. The increase in protein content can be accounted for, at least in part by increases in certain specific, electrophoretically distinct, protein fractions. Similar changes were observed with each of the four varieties examined.