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Modification of wheat gliadin by γ‐rays
Author(s) -
Booth M. R.
Publication year - 1970
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740210310
Subject(s) - gliadin , chemistry , sephadex , gluten , starch , macromolecule , electrophoresis , amino acid , polysaccharide , chromatography , carbohydrate , biochemistry , enzyme
Wheat gliadin has been γ‐irradiated under nitrogen with a dose of 20 Mrad. No significant changes were observed in the amino acid analysis, chymotryptic fingerprints, or elution curves from Sephadex G‐200. Discrete bands had been largely replaced by tailing in the starch‐gel electrophoretic pattern. It is concluded that random bond scission has occurred, chiefly altering the side chains, and to some degree protein fragments have been grafted on to other sites. There is no definite evidence for the formation of macromolecular structures by inter‐protein crosslinks. On average it is likely that of the order of only one or two bonds per molecule were affected.