Premium
Reduction and RE‐oxidation of the purothionins
Author(s) -
Redman D. G.,
Elton G. A. H.
Publication year - 1969
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740200908
Subject(s) - acrylonitrile , chemistry , intermolecular force , starch , electrophoresis , polymer chemistry , chromatography , organic chemistry , polymer , molecule , copolymer
Abstract The disulphide bonds of the purothionins have been fully reduced with 2‐mercaptoethanol. Starch‐gel electrophoresis of the products and also of the reduced proteins after blocking with acrylonitrile confirms previous tentative evidence that purothionins a and 0 both consist of single polypeptide chains. Reduced purothionin doublet has been re‐oxidised under various conditions, the major finding being that in dilute solution re‐formation of structure predominates whereas in very concentrated solution, extensive intermolecular cross linking appears to occur.