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Fractionation of plant material II. —Amino acid composition of some fractions obtained from the broad bean plant ( Vicia faba L.) and chromatographic differentiation of hydroxyproline isomers
Author(s) -
Clarke Eileen M. W.,
Ellinger Gabrielle M.
Publication year - 1967
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740181109
Subject(s) - hydroxyproline , vicia faba , proline , chemistry , fractionation , chromatography , amino acid , acetic acid , composition (language) , extraction (chemistry) , botany , organic chemistry , biochemistry , biology , linguistics , philosophy
The amino acid compositions of three protein fractions obtained from broad bean leaves were estimated by ion exchange chromatography. Hydroxyproline was found present in the TCA‐soluble, non‐diffusible fraction and in the final residue after the further extraction with phenol‐acetic acid‐water (2: 1 : 1, w/v/v), but not in the extract itself. By comparison with the behaviour of four authentic isomers of hydroxyproline, under two standard conditions of chromatographic analysis, the isomer found was trans ‐4‐hydroxy‐proline. These results are discussed in relation to other reports of hydroxyproline occurring in plant material.