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Effect of optical systems and sample preparation on the visible reflection spectra of pork muscle
Author(s) -
Elliott R. J.
Publication year - 1967
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740180802
Subject(s) - reflectivity , materials science , spectral line , diffuse reflection , reflection (computer programming) , optics , chemistry , analytical chemistry (journal) , chromatography , physics , astronomy , computer science , programming language
The technique for measuring the reflectance spectrum over the visible range of pork muscle, as influenced by optical design and sample preparation, was examined. Of the three optical systems used to measure light reflectance from muscle, the best resolution of the spectra was achieved with integration spheres. The influence on the reflection spectra of the following factors was investigated: muscle surface, transparent film, depth of sample, longitudinal and transverse sections of muscle fibres, and the effect of fat. The spectra of samples within the longissimus dorsi muscle was affected by pH, but not by vertebral location. A thin sample covered with a glass cover slip and with a white rear reflector produced the maximum resolution of muscle pigments.