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A silica gel chromatographic procedure adapted to liquid‐scintillation counting of 14 c labelled organic acids from plant material and silage
Author(s) -
Lessard J. R.,
McDonald P.
Publication year - 1966
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740170607
Subject(s) - chromatography , chemistry , elution , silica gel , citric acid , oxalic acid , extraction (chemistry) , fumaric acid , organic acid , ion exchange , silage , organic chemistry , ion , food science
A method is described for the separation of a wide range of organic acids present in plant material and in silage. The method consists of cold extraction with dilute sulphuric acid, followed by purification of the extract on a silica gel column. The organic acids in the purified extract were separated quantitatively using a continuous gradient‐elution system of eluents through a small silica gel column. The extraction method was found to be more efficient for extracting citric, oxalic and fumaric acids from red clover, than a standard method with ethanol, water and ion‐exchange resins. Most of the acids of the tricarboxylic acid cycle were detected in red clover. An unidentified acid accounted for more than 37% of the organic acidity. The activity of 14 C‐labelled acids could be measured directly on the eluate from the column in combination with a liquid scintillation system.