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The isolation, properties, and enzymic breakdown of linamarin from cassava
Author(s) -
Wood T.
Publication year - 1966
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740170209
Subject(s) - glucoside , isolation (microbiology) , chemistry , chromatography , manihot esculenta , euphorbiaceae , botany , biology , pathology , medicine , alternative medicine , microbiology and biotechnology
A new method for the isolation of linamarin from Cassava is described. Using the pure glucoside, its properties and assay were investigated and a number of modifications to the previous assay procedure were introduced. The paper chromatography of the glucoside and means of detecting it on chromatograms are described. A procedure for preparing and assaying a crude preparation of linamarase is given. The variation in activity with pH, the stability of the enzyme, and its inhibition by buffers and by (1·5)‐gluconolactone are described. A suggestion is made as to the role of linamarin and linamarase in the plant.