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Estimation of nisin in foods
Author(s) -
Tramer J.,
Fowler G. G.
Publication year - 1964
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740150802
Subject(s) - nisin , food science , spore , chemistry , chromatography , incubation , microbiology and biotechnology , biology , biochemistry , antimicrobial
Methods for the estimation of nisin in solution and in foods are described and the nisin unit is redefined. The presence of interfering substances in certain food extracts has been demonstrated. In the foods examined compensation for the effect on the plate assay could be made by inactivating the nisin by an alkali treatment, and using these treated extracts as a basis for controls. For quantitative determination of suspensions of nisin alone and in food extracts, a large plate diffusion method requiring no prediffusion and using M. flavus as test organism is described. For semi‐quantitative estimations, or for sorting purposes, a simple reverse‐phase disk assay technique based on B. stearothermophilus spores, with overnight incubation at 55°, is proposed.