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Comparison of chemical and bioassay estimates of oestrogenic potency of clover
Author(s) -
Flux D. S.,
Wilson G. F.,
Wong E.
Publication year - 1964
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740150611
Subject(s) - bioassay , formononetin , biochanin a , potency , genistein , biological activity , biology , chemistry , pharmacology , daidzein , endocrinology , biochemistry , in vitro , genetics
The oestrogenic potencies of red and subterranean clover extracts, the isoflavone contents of which have previously been determined by chemical means, have been estimated by bioassay, and results from the two different methods are compared. Bioassay studies showed that there is no interaction between biochanin A and genistein or biochanin A and formononetin in their effects on mouse uteri. Pratensein has oestrogenic activity in that it causes an increase in uterine weight in immature ovariectomised mice.