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Apparatus for automatic elution of wheat‐protein fractions during starch‐gel electrophoresis
Author(s) -
Elton G. A. H.,
Ewart J. A. D.
Publication year - 1963
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740140308
Subject(s) - chromatography , fraction (chemistry) , starch , electrophoresis , two dimensional gel electrophoresis , gel electrophoresis , chemistry , elution , gel electrophoresis of proteins , free flow electrophoresis , polyacrylamide gel electrophoresis , biochemistry , proteomics , enzyme , gene
Vertical starch‐gel electrophoresis of wheat proteins is carried out in an apparatus in which a buffer‐filled slot in the gel is flushed at intervals into a fraction collector. The protein fractions obtained are very sharp, sometimes running as single bands upon further starch‐gel electrophoresis.