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Plant lipids. II. —Free and combined sterols (sterol esters and glycosides) in commercial oilseed phospholipids
Author(s) -
Aylward F.,
Nichols B. W.
Publication year - 1962
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740130204
Subject(s) - sterol , glycoside , stigmasterol , chemistry , chromatography , campesterol , phospholipid , glucoside , silicic acid , biochemistry , cholesterol , organic chemistry , membrane , medicine , alternative medicine , pathology
An acetone extraction method for the isolation of free and combined sterols has been applied to commercial phospholipid fractions from groundnut, rapeseed and linseed; all three lipid samples contained 1‐3% of sterol glycosides. The glycoside from groundnut phospholipids was composed mainly of β‐sitosterol‐D‐glucoside, with considerably smaller quantities of glucosides of stigmasterol and a saturated sterol. The non‐glycoside sterols from the same source had a very similar composition to those from the glucosides. Washing with cold acetone, dialysis through a rubber membrane and chromatography on silicic acid were all efficient in removing free and esterified sterols from crude phospholipids, but were less effective in removing sterol glycosides.