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Extraction of proteins from green leaves
Author(s) -
Festenstein G. N.
Publication year - 1961
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740120409
Subject(s) - extraction (chemistry) , maceration (sewage) , nitrogen , chemistry , chloroplast , dispersion (optics) , chromatography , botany , biology , materials science , biochemistry , organic chemistry , composite material , physics , optics , gene
Extraction of nitrogenous material (especially protein nitrogen) from leaves of different species has been studied using the Pirie steel press, which grinds small quantities of leaves by forcing them through a narrow slot. Extraction of young tobacco leaves is comparable with high‐speed maceration and sedimentable material is more dispersed; increased extraction under alkaline conditions is largely due to greater dispersion of chloroplasts, appreciable at 1500 g, but only slight at 12,000 g; a total of 90–95% extraction of nitrogenous material is obtained with the use of detergents to release nitrogen from residual fibre after initial extraction in the press. Comparatively less nitrogen is released from macerated fibre.