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The micro‐determination of glycerol in Acetobacter culture media
Author(s) -
Jackson C. P.,
Ramamurti K.
Publication year - 1958
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740091204
Subject(s) - glycerol , dihydroxyacetone , acetobacter , chemistry , biochemistry , chromatography , fermentation
A method is presented for the colorimetric estimation of glycerol in the presence of the metabolism products which it yields when submitted to the action of Acetobacter acetigenum. The interference of dihydroxyacetone (DHA) is eliminated by selective oxidation with ceric sulphate and hypoiodite. The method is suitable for multiple routine analyses and allows 0.1‐0.5 mg. of glycerol to be determined accurately in the presence of 0.76 mg. of DHA. The precision of the method is related to the accuracy of prediction, from standard curves, of the glycerol content of unknown solutions, for which 95% confidence limits at the extreme values of the determinative range are given as 0.1 ± 0.01 mg. and 0.5 ± 0.011 mg.