Premium
A rapid procedure for the chromatographic separation and spectrophotometric estimation of certain pasture lipoids. I.—Carotene, xanthophyll and chlorophyll
Author(s) -
Workker N. A.
Publication year - 1957
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740080713
Subject(s) - xanthophyll , pasture , chromatography , extraction (chemistry) , carotene , chlorophyll , acetone , chemistry , chlorophyll a , botany , biology , agronomy , carotenoid , biochemistry
A method is described for the extraction and chromatographic separation of carotene, xanthophyll and chlorophyll from pasture plants. Species examined in the present investigations included ryegrass, cocksfoot, red and white clovers, but there is no reason why the method should not be used for examining other pasture or crop plants The pigments are extracted with acetone, transferred to light petroleum, chromatographed on alumina into three fractions and then estimated spectrophotometrically. The method is rapid and accurate and is suitable as a routine assay procedure.