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Quantitative determinations of amino‐acids in undesalted hydrolysates by buffer filter‐paper chromatography
Author(s) -
Baker B. E.,
Khan N. A.
Publication year - 1957
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740080404
Subject(s) - chromatography , amino acid , hydrolysate , glycine , chemistry , casein , filter paper , arginine , solvent , paper chromatography , hydrolysis , biochemistry
Abstract The amino‐acids of undesalted casein hydrolysates have been determined successfully by quantitative chromatography on buffered filter paper. The technique proposed includes the use of five newly developed solvent systems. The values for the amino‐acid content of casein found by this technique were in agreement with the values reported in the literature. The coefficients of variation for the determination of individual amino‐acids ranged from 7.4% for glycine to 1.3% for arginine.