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Manufacture of algal chemicals. VII. —the isolation of algal carbohydrated by means of charcoal columns
Author(s) -
Black W. A. P.,
Dewar E. T.,
Woodward F. N.
Publication year - 1955
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740061205
Subject(s) - charcoal , chromatography , hydrolysate , chemistry , fucose , hydrolysis , laminarin , isolation (microbiology) , acid hydrolysis , salt (chemistry) , elution , biochemistry , biology , organic chemistry , microbiology and biotechnology , glycoprotein
Charcoal columns have been examined as a means of separating D‐glucose and L‐fucose obtained on acid hydrolysis of brown seaweeds. Glucose and fucose can be isolated on the one‐gramme scale from synthetic mixtures and from hydrolysates of appropriate seaweeds on a column (46 × 5.0 cm.) with water as eluant; but difficulties are encountered on scaling up the process. Demineralization of mannitol‐salt mixtures and the isolation of laminaribiose from partially hydrolysed laminarin can also be effected on charcoal columns.