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The determination of tocopherols by paper chromatography
Author(s) -
Green J.,
Marcinkiewicz S.,
Watt P. R.
Publication year - 1955
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740060506
Subject(s) - chromatography , chemistry , tocopherol , vitamin e , chromatographic separation , zinc , high performance liquid chromatography , antioxidant , organic chemistry
Methods are given for determining individual tocopherols in natural oils and food‐stuffs. These methods consist of either a single‐dimensional separation on zinc carbonate‐impregnated paper, or this separation in conjunction with a second‐dimensional partition separation on paraffin‐coated paper. Chromatographic runs are short and rapid and can be made without preliminary removal of carotenoids and moderate amounts of vitamin A or sterols. Over 98% recoveries are obtained with pure tocopherols. The presence of the recently described 5‐methyltocol in wheat products has been confirmed, and, in addition, using two‐dimensional methods, a substance has been isolated from some cereal grains, which appears to be a new tocopherol.