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The use of an ion‐exchange resin for tissue hydrolysis in the determination of hexosamine
Author(s) -
Anastassiadis P. A.,
Common R. H.
Publication year - 1955
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740060409
Subject(s) - hydrolysis , ion exchange resin , elution , chemistry , hydrochloric acid , chromatography , ion exchange , hexosamines , ion , organic chemistry , glucosamine
Hydrolysis of tissue samples with Dowex‐50 resin suspended in 0.05N‐hydrochloric acid, with subsequent elution of the hexosamine from the resin, afforded water‐clear solutions containing the hexosamine. The hexosamine in this eluate could be determined directly by a modification of the method of Elson & Morgan, thus reducing or avoiding the necessity of correcting for interference by interactions between sugars and amino‐acids and by humin. The technique described gave results about 10% lower than those obtained by the modified method previously described by the authors.