Analytical studies on the carbohydrates of grasses and clovers. V. —Development of a method for the estimation of cell‐wall polysaccharides | Zendy

Harwood V. D. | Zendy

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Analytical studies on the carbohydrates of grasses and clovers. V. —Development of a method for the estimation of cell‐wall polysaccharides

Author(s) -

Harwood V. D.

Publication year - 1954

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2740050603

Subject(s) - hemicellulose , hydrolysis , polysaccharide , chemistry , residue (chemistry) , acid hydrolysis , cellulose , sugar , cell wall , carbohydrate , fraction (chemistry) , alcohol , chromatography , food science , biochemistry , organic chemistry

The cell‐wall polysaccharides, i.e. the carbohydrate fraction of herbage that is insoluble in alcohol and cold water, are determined by a two‐stage acid‐hydrolysis procedure. In the first stage, treatment with N‐sulphuric acid effects solution and hydrolysis of the greater part of the hemicellulose fraction, with a maximum variability in any one sugar of 7% of the total hydrolysed. The remaining ‘cellulose’ residue is saccharified with 72% sulphuric acid during the second stage, with agreement within 5%.

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