Premium
Analytical studies on the carbohydrates of grasses and clovers. V. —Development of a method for the estimation of cell‐wall polysaccharides
Author(s) -
Harwood V. D.
Publication year - 1954
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740050603
Subject(s) - hemicellulose , hydrolysis , polysaccharide , chemistry , residue (chemistry) , acid hydrolysis , cellulose , sugar , cell wall , carbohydrate , fraction (chemistry) , alcohol , chromatography , food science , biochemistry , organic chemistry
The cell‐wall polysaccharides, i.e. the carbohydrate fraction of herbage that is insoluble in alcohol and cold water, are determined by a two‐stage acid‐hydrolysis procedure. In the first stage, treatment with N‐sulphuric acid effects solution and hydrolysis of the greater part of the hemicellulose fraction, with a maximum variability in any one sugar of 7% of the total hydrolysed. The remaining ‘cellulose’ residue is saccharified with 72% sulphuric acid during the second stage, with agreement within 5%.