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Procedures for the extraction, separation and estimation of the major fat‐soluble pigments of hay
Author(s) -
Davidson J.
Publication year - 1954
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740050101
Subject(s) - xanthophyll , chromatography , pigment , extraction (chemistry) , chemistry , chlorophyll , chlorophyll a , carotene , saponification , hay , acetone , spectrophotometry , chloroform , chlorophyll b , botany , biochemistry , food science , biology , organic chemistry
New methods are described for the extraction, chromatographic separation and spectro‐photometric identification and estimation of chlorophyll‐ a , chlorophyll‐ b , phaeophytin‐ a , phaeophytin‐ b , carotene and xanthophyll in hay. The most suitable solvent for extraction was 85% acetone. The chlorophylls were separated chromatographically from the phaeophytins, carotene and xanthophyll on a sucrose‐sodium sulphate mixture. The chlorophyll‐ a and chlorophyll‐ b were estimated simultaneously in the green solution, and phaeophytin‐ a and phaeophytin‐ b were estimated simultaneously in the yellow solution, by spectrophotometry. Carotene and, subsequently, xanthophyll were separated chromato‐graphically from the yellow solution on magnesium oxide, and were estimated separately.