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The estimation of vitamin‐E activity by paper chromatography
Author(s) -
Eggitt P. W. Russell,
Ward L. D.
Publication year - 1953
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2740040404
Subject(s) - chromatography , petroleum ether , elution , chemistry , filter paper , inert , paper chromatography , inert gas , gas chromatography , column chromatography , vaseline , ethyl acetate , organic chemistry , biology , wound healing , immunology , extraction (chemistry)
Paper chromatography has recently been applied to the separation and estimation of the individual tocopherols. The present communication draws attention to errors that are likely to arise if a solution of ‘Vaseline’ petroleum jelly in ethyl ether is used to impregnate the filter paper in order to achieve phase‐reversal. The use of liquid paraffin B.P., dissolved in light petroleum, not only overcomes these objections, but enables the elution after chromatography to be simplified. Losses of tocopherols are minimized by chromatography in an inert atmosphere. The modified technique, which is described in detail, has consistently given recovery figures of 95%, or better, when tested with mixtures of synthetic tocopherols.