Isolation of eriocitrin (eriodictyol 7‐ O ‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit ( Citrus lumia ) and its distribution in  Citrus  species | Zendy

Nogata Yoichi | Zendy; Ohta Hideaki | Zendy; Ishii Toshinao | Zendy; Sekiya Keizo | Zendy

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Isolation of eriocitrin (eriodictyol 7‐ O ‐rutinoside) as an arachidonate lipoxygenase inhibitor from Lumie fruit ( Citrus lumia ) and its distribution in Citrus species

Author(s) -

Nogata Yoichi,

Ohta Hideaki,

Ishii Toshinao,

Sekiya Keizo

Publication year - 2006

Publication title -

journal of the science of food and agriculture

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.782

H-Index - 142

eISSN - 1097-0010

pISSN - 0022-5142

DOI - 10.1002/jsfa.2674

Subject(s) - eriodictyol , lipoxygenase , chemistry , biochemistry , arachidonate 5 lipoxygenase , naringin , enzyme , naringenin , flavonoid , chromatography , arachidonic acid , antioxidant

An inhibitory compound acting against rat platelet 12‐lipoxygenase was isolated from the peel of Lumie fruit ( Citrus lumia ) by activity‐guided separation. It was identified as eriocitrin (eriodictyol 7‐ O ‐rutinoside) by spectroscopic analyses. Eriocitrin inhibited 5‐lipoxygenase (IC 50 29.1 µmol L −1 ) from rat peritoneal polymorphonuclear leukocytes in addition to 12‐lipoxygenase (IC 50 22.3 µmol L −1 ). Its aglycone, eriodictyol (5,7,3′, 4′‐tetrahydroxyflavanone), was a much more potent inhibitor of both 12‐lipoxygenase (IC 50 0.07 µmol L −1 ) and 5‐lipoxygenase (IC 50 0.20 µmol L −1 ). It also inhibited the production of leukotriene B 4 in intact peritoneal polymorphonuclear leukocytes stimulated with calcium ionophore A23187 (IC 50 12.7 µmol L −1 ). The distribution of eriocitrin in 39 citrus fruits was investigated by high‐performance liquid chromatography analysis. Lumie, eureka lemon ( Citrus limon ), Sambokan ( Citrus sulcata ), Sudachi ( Citrus sudachi ) and Koji ( Citrus leiocarpa ) fruits were found to contain high levels of eriocitrin in both peel and juice vesicles. Copyright © 2006 Society of Chemical Industry

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