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Methodology for the determination of biological antioxidant capacity in vitro : a review
Author(s) -
MacDonaldWicks Lesley K,
Wood Lisa G,
Garg Manohar L
Publication year - 2006
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2603
Subject(s) - antioxidant , in vitro toxicology , antioxidant capacity , biochemical engineering , in vitro , microbiology and biotechnology , reliability (semiconductor) , in vivo , food science , chemistry , biology , biochemistry , engineering , power (physics) , physics , quantum mechanics
One of the effective means of adding value to agricultural produce is the development of functional foods or dietary supplements enriched with antioxidants. This process involves extraction of antioxidant rich fractions from their sources and then testing for antioxidant activity prior to adding these back to the foods or packing into pills or capsules. Ideally, the antioxidant activity should be tested using both in vitro and in vivo techniques but due to the high cost of conducting animal and human feeding trials, many products undergo only in vitro testing. This review describes popular methods commonly used for testing antioxidant activity in vitro , with particular reference to their reliability, efficiency, accessibility and biological relevance. Copyright © 2006 Society of Chemical Industry