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Application of flow nephelometry to the analysis of the influence of carbohydrates on protein–tannin interactions
Author(s) -
Carvalho Elisabete,
Póvoas Maria João,
Mateus Nuno,
de Freitas Victor
Publication year - 2006
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2430
Subject(s) - tannin , polysaccharide , chemistry , xanthan gum , bovine serum albumin , nephelometry , dextran , food science , chromatography , proanthocyanidin , biochemistry , polyphenol , rheology , antioxidant , biology , materials science , antibody , immunology , composite material
The flow nephelometric technique was recently introduced to study the interaction between tannins and proteins. This technique was employed here to study the ability of the polysaccharides xanthan, dextran and gum arabic to disrupt the interactions between condensed tannin fractions from grape seed and bovine serum albumin (BSA). Xanthan was shown to have a higher ability to inhibit the aggregate formation of tannins and BSA than the other polysaccharides. Xanthan's ability to inhibit protein‐tannin complex formation was higher for condensed tannin fractions of smaller size and lower complexity. Copyright © 2006 Society of Chemical Industry