Premium
Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin β‐ODAP (β‐ N ‐oxalyl‐ L ‐α,β‐diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system
Author(s) -
Marichamy Sankar,
Yigzaw Yirgalem,
Gorton Lo,
Mattiasson Bo
Publication year - 2005
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2211
Subject(s) - clostridium , horseradish peroxidase , rumen , bacteria , chromatography , chemistry , obligate anaerobe , enzyme , biochemistry , biology , fermentation , genetics
Six pure strains of obligate anaerobes capable of degrading the toxin β‐ N ‐oxalyl‐ L ‐α, β‐diaminopropionic acid (β‐ODAP) contained in grass pea ( Lathyrus sativus ) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The β‐ODAP degrading efficiency of the isolates was evaluated by measuring the amount of β‐ODAP in the growth medium, which contained β‐ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on co‐immobilising two enzymes, glutamate oxidase (GlOx) and horseradish peroxidase (HRP), on the end of a spectrographic graphite electrode. β‐ODAP is oxidised by GlOx to form H 2 O 2 , which in turn is bioelectrocatalytically reduced by HRP through a mediated reaction using a polymeric mediator incorporating Os 2 + /3+ functionalities rapidly shuttling electrons with the electrode_giving rise to the analytical signal. On the basis of this analysis system, the new isolates are capable of utilising β‐ODAP as sole carbon source to a maximum of 90–95% within 5 days with concomitant increase in cell protein. Copyright © 2005 Society of Chemical Industry