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Positional analysis of egg triacylglycerols and phospholipids from hens fed diets enriched with refined seal blubber oil
Author(s) -
Pacetti Deborah,
Hulan Howard W,
Schreiner Matthias,
Boselli Emanuele,
Frega Natale G
Publication year - 2005
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2174
Subject(s) - polyunsaturated fatty acid , blubber , phosphatidylcholine , composition (language) , food science , phosphatidylethanolamine , biology , fatty acid , chemistry , biochemistry , phospholipid , ecology , linguistics , philosophy , membrane
The effect of feeding laying hens with refined seal blubber oil (SBO, containing 22.2% ω3 polyunsaturated fatty acids) on the fatty acids composition and regiospecific distribution of fatty acids in triacylglycerols (TAG) and phospholipids (PL) of egg produced was investigated. The hens were fed four diets containing 0 (control), 1.25, 2.5 or 5% SBO for 5 and 9 weeks. Comparison of the total fatty acids composition indicated that a 5 week feeding period was sufficient to obtain the highest amount of total polyunsaturated fatty acids in the eggs. The fatty acids composition of the egg lipids reflected the fatty acids composition of the diet. The substantial reduction in the ω6/ω3 ratio (ie 8.5 to 2.6) is of interest, and is in line with the recommendations of health authorities in several countries, where it has been suggested that the human diet should contain a ω6/ω3 ratio of 3–4. The greatest reduction of the ω6/ω3 ratio occurred for diet A (control) and diet B (1.25% SBO). Principal component analysis analysis of the fatty acids composition of egg lipid showed four clusters representing: (i) the control diet; (ii) the diet containing 1.25% SBO as well as samples obtained from feeding 2.5% SBO for 9 weeks; (iii) the diet with 5% SBO; and (iv) samples obtained from hens fed 2.5% SBO for 5 weeks. More ω‐3 polyunsaturated fatty acids (ω3 PUFA) were incorporated in phosphatidylethanolamine and phosphatidylcholine than in TAG. Although ω3 were predominantly linked in the position Sn‐1,3 of TAG in SBO, they were esterified in the Sn‐2 position of the TAG and PL of eggs obtained after feeding laying hens with enriched diets. Copyright © 2005 Society of Chemical Industry