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Antioxidant activity and characterization of volatile constituents of beechwood creosote
Author(s) -
Lee KwangGeun,
Lee SungEun,
Takeoka Gary R,
Kim JeongHan,
Park ByeoungSoo
Publication year - 2005
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2156
Subject(s) - creosote , chemistry , hexanal , antioxidant , chromatography , phenol , gas chromatography , phenols , guaiacol , gas chromatography–mass spectrometry , food science , organic chemistry , mass spectrometry , enzyme
Volatile constituents of beechwood creosote were determined using gas chromatography (GC) and gas chromatography–mass spectrometry (GC‐MS). The major volatile constituents of creosote were 2‐methoxyphenol (guaiacol; 25.2%) 2‐methoxy‐4‐methylphenol (4‐methylguaiacol; 21.4%), 3‐methylphenol ( m ‐cresol; 8.3%) 4‐methylphenol ( p ‐cresol; 7.9%) 2‐methylphenol ( o ‐cresol; 4.6%) and phenol (2.8%). The antioxidant activity of creosote was evaluated by three different chemical assays. Beechwood creosote exhibited potent inhibitory effects on the formation of conjugated diene hydroperoxides (from methyl linoleate) at concentrations of 500, 750 and 1000 µg ml −1 . Creosote had a potent inhibitory effect on the oxidation of hexanal for 40 days at a level of 5 µg ml −1 and also inhibited malonaldehyde (MA) formation from ethyl arachidonate by 92% at a level of 50 µg ml −1 . The antioxidative activity of creosote was comparable with that of the well‐known antioxidants α‐tocopherol and BHT in the hexanal assay. However, creosote displayed comparatively lower antioxidant activity in the other two assays. Copyright © 2005 Society of Chemical Industry

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