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Isolation of a free radical‐scavenging antioxidant from water spinach ( Ipomoea aquatica Forsk)
Author(s) -
Prasad K Nagendra,
Divakar Soundar,
Shivamurthy Gyarahally R,
Aradhya Somaradhya Mallikarjuna
Publication year - 2005
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.2125
Subject(s) - ipomoea aquatica , chemistry , antioxidant , spinach , fractionation , chromatography , silica gel , ipomoea , scavenging , lipid peroxidation , column chromatography , liposome , flavonoid , food science , organic chemistry , biochemistry , botany , biology
Ipomoea aquatica Forsk, a green leafy vegetable that is a rich source of vitamins and amino acids with many health benefits, has been explored for the isolation and identification of its bioactive compounds. Activity‐guided repeated fractionation of a methanol extract on a silica gel column followed by an XAD column yielded a compound that exhibited antioxidant activity with an EC 50 value of 83 ± 1.02 µg ml −1 reaction mixture. It also showed very strong lipid peroxidation‐inhibitory activity in a liposome model system with an EC 50 value of 72.2 ± 0.9 µg ml −1 . However, it showed negligible metal‐chelating activity. Based on UV, 2D nuclear magnetic resonance and gas chromatography/mass spectrometry studies, the compound was tentatively identified to be 7‐ O ‐β‐ D ‐glucopyranosyl‐dihydroquercetin‐3‐ O ‐α‐ D ‐glucopyranoside. This is the first report on the antioxidant properties of I aquatica leaf extracts. Copyright © 2005 Society of Chemical Industry