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Antioxidant activity of Potentilla fruticosa
Author(s) -
Miliauskas Giedrius,
van Beek Teris A,
Venskutonis Petras R,
Linssen Jozef P H,
de Waard Pieter,
Sudhölter Ernst JR
Publication year - 2004
Publication title -
journal of the science of food and agriculture
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.782
H-Index - 142
eISSN - 1097-0010
pISSN - 0022-5142
DOI - 10.1002/jsfa.1914
Subject(s) - chemistry , abts , dpph , quercetin , ellagic acid , kaempferol , oxygen radical absorbance capacity , antioxidant , catechin , flavonols , organic chemistry , chromatography , polyphenol
Abstract The molecular structures of the radical scavenging compounds present in extracts of Potentilla fruticosa blossoms were elucidated and the antioxidant activities of various extracts were determined. The activities of the different fractions were monitored by off‐line and on‐line RP‐HPLC DPPH • and ABTS •+ scavenging methods. Twelve compounds were isolated and identified, namely ellagic acid, catechin, quercetin‐3‐β‐glucopyranoside, quercetin‐3‐β‐galactopyranoside, quercetin‐3‐β‐rutinoside, quercetin‐3‐β‐glucuronopyranoside, quercetin‐3‐α‐arabinofuranoside, kaempferol‐3‐β‐rutinoside, kaempferol‐3‐ O ‐β‐(6″‐ O ‐( E )‐ p ‐coumaroyl)glucopyranoside, rhamnetin‐3‐β‐glucopyranoside and rhamnetin‐3‐β‐galactopyranoside. The radical scavenging activity of each isolated compound was measured using DPPH • and ABTS •+ assays and compared with the activity of rosmarinic acid. Catechin and ellagic acid were found to be the most active radical scavengers. The antioxidant properties of plant fractions were assessed in model systems by measuring superoxide anion and hydrogen peroxide scavenging, β‐carotene bleaching, hexanal production in edible oil, peroxide formation, and the increase in UV absorbance in the course of oxidation. Copyright © 2004 Society of Chemical Industry